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M94A3222.TXT
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1994-10-25
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Document 3222
DOCN M94A3222
TI Interleukin 12 enhances host responses of HIV positive donors against
Mycobacterium avium in vitro.
DT 9412
AU Newman G; Guarnaccia J; Vance E; Remold H; Kazanjian P; Dept.
Rheum/Immunol, Brigham & Women's Hospital/Harvard Medical; School,
Boston, MA 02115.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):125 (abstract no. PA0121). Unique
Identifier : AIDSLINE ICA10/94369353
AB OBJECTIVE: To determine whether IL-12 could enhance growth inhibition of
an proliferative responses to M. avium by peripheral blood mononuclear
cells (PMBC) from either HIV negative or positive donors. METHODS: PBMCs
from 14 HIV+ and 12 HIV- donors were inoculated with M. avium, serovar
4, and cultured for 7 days in the presence of IL-12. The cells were
lysed and the amount of M. avium present determined by a radiometric
broth assay. PBMCs from these same donors and 10 additional HIV+ donors,
were also analyzed for their ability to proliferate to M. avium in the
presence or absence of IL-12 by 3H-thymidine uptake. RESULTS: IL-12
significantly enhanced (p = .001) the capacity of PBMCs to inhibit the
growth of M. avium from all 12 HIV- donors, but only 5 of 14 HIV+
donors. in contrast, IL-12 significantly (p = .0001) stimulated
increased proliferation to M. avium by all donors regardless of HIV
infection, although HIV- donors had a significantly greater response (p
= .008) than HIV+ donors. IL-12 enhanced proliferation by HIV+ donors to
M. avium was independent of CD4 counts. CONCLUSIONS: Exogenously
administered IL-12 enhanced in vitro cellular responses to M. avium in
both HIV+ and - donors. These results suggest that IL-12 by itself or
used in conjunction with other cytokines may be of therapeutic value
against M. avium infections in AIDS patients.
DE Cells, Cultured Comparative Study Growth Substances/*PHARMACOLOGY
Human HIV Seronegativity HIV Seropositivity/*IMMUNOLOGY
Interleukins/*PHARMACOLOGY Lymphocyte Transformation/DRUG EFFECTS
Lymphocytes/*DRUG EFFECTS/IMMUNOLOGY/MICROBIOLOGY Mycobacterium
avium/*DRUG EFFECTS/GROWTH & DEVELOPMENT Reference Values
Thymidine/METABOLISM MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).